Pepsin is widely used in the food industry for the production of protein hydrolysates, which are essential in creating dietary supplements, infant formulas, and sports nutrition products. Its ability to break down proteins into smaller peptides makes it valuable in cheese manufacturing, where it aids in curdling milk to produce cheese.

In the medical field, pepsin is utilized as a digestive enzyme in medications to treat conditions like indigestion and pancreatic insufficiency, helping individuals with impaired digestion to better absorb nutrients.

Additionally, it finds application in laboratory research for protein analysis and tissue dissociation in cell culture studies. Its specificity in cleaving peptide bonds makes it a crucial tool in biochemistry and molecular biology experiments.