This compound is primarily utilized in the field of medical research, particularly in the study of enzyme inhibition. It acts as a potent inhibitor of dihydrofolate reductase (DHFR), an enzyme crucial for DNA synthesis and cell proliferation. By inhibiting DHFR, it disrupts the folate metabolism pathway, making it valuable in the development of anticancer and antimicrobial agents. Its application extends to the investigation of drug resistance mechanisms in various pathogens, aiding in the design of more effective therapeutic strategies. Additionally, it serves as a key intermediate in the synthesis of more complex molecules, such as methotrexate, used in targeted drug delivery systems and chemotherapy.