Methylene Blue is widely used in the medical field as a dye and treatment. It is commonly employed as a staining agent in histology to highlight cellular structures under a microscope, aiding in the diagnosis of various conditions. In medicine, it is used to treat methemoglobinemia, a condition where the blood cannot carry oxygen effectively, by converting methemoglobin back to hemoglobin. Additionally, it has been explored as a treatment for urinary tract infections and as an antidote for cyanide poisoning. In surgery, it is used to mark surgical sites or visualize blood vessels and lymphatic pathways. In research, Methylene Blue is utilized in studies related to mitochondrial function and oxidative stress due to its ability to act as an electron carrier. It also finds application in aquaculture as an antifungal and antiparasitic agent to treat fish diseases, and as an antimicrobial in certain cases.