Erythromycin estolate is widely used in the treatment of various bacterial infections, particularly those caused by susceptible strains of microorganisms. It is commonly prescribed for respiratory tract infections, including bronchitis and pneumonia, as well as skin and soft tissue infections. Additionally, it is effective in treating certain sexually transmitted infections like syphilis and chlamydia.

In pediatric care, erythromycin estolate is often chosen for its palatable liquid formulation, making it easier to administer to children. It is also used as an alternative for patients allergic to penicillin.

Beyond its antimicrobial applications, erythromycin estolate is sometimes utilized in the management of gastrointestinal motility disorders due to its prokinetic effects, although this is not its primary indication.

Its broad-spectrum activity and relatively low toxicity make it a valuable option in clinical practice, especially in cases where other antibiotics may be less effective or contraindicated.