Recombinant trypsin is widely used in cell culture applications, especially in the detachment of adherent cells during subculturing. It offers a highly specific and consistent alternative to animal-derived trypsin, reducing the risk of contamination from viruses or prions. Its use is critical in biopharmaceutical manufacturing, including the production of monoclonal antibodies and vaccines, where process consistency and regulatory compliance are essential. It is also employed in proteomics for protein digestion prior to mass spectrometry analysis, providing reproducible cleavage at lysine and arginine residues. Due to its high purity and batch-to-batch reliability, recombinant trypsin is preferred in diagnostic kits and regenerative medicine, including stem cell culture workflows.