Recombinant trypsin is widely used in cell culture applications, particularly for cell detachment during subculturing. It efficiently breaks down proteins that anchor cells to the surface of culture vessels, allowing gentle and consistent cell harvesting without damaging cell membranes when used at optimal concentrations. It is preferred over animal-derived trypsin due to its high purity, reduced risk of contamination with viruses or prions, and batch-to-batch consistency. This makes it ideal for use in therapeutic cell production, including stem cell cultures and biopharmaceutical manufacturing. Recombinant trypsin is also employed in proteomics research for protein digestion prior to mass spectrometry analysis. Its high specificity for cleaving peptide bonds at lysine and arginine residues ensures reproducible and reliable results in protein identification and characterization. Additionally, it finds application in diagnostic assays and enzyme-based detection systems where precise and controlled proteolytic activity is required. Its recombinant nature supports compliance with regulatory standards in clinical and pharmaceutical settings.