Recombinant trypsin is widely used in cell culture applications, particularly for cell detachment during subculturing. It efficiently breaks down proteins that anchor cells to the surface of culture vessels, allowing gentle and consistent cell harvest without damaging cell membranes. Due to its high purity and absence of animal-derived components, it reduces the risk of contamination and improves reproducibility in sensitive applications like stem cell culture, vaccine production, and biopharmaceutical manufacturing.

It is also employed in proteomics for protein digestion prior to mass spectrometry analysis, offering precise cleavage at lysine and arginine residues.

Additionally, in recombinant protein production, recombinant trypsin is used to cleave specific peptide sequences to activate target proteins or remove purification tags, yielding a final product with structure and function closely resembling the natural protein.

Its consistent activity and batch-to-batch reliability make it ideal for automated and large-scale processes in both research and industrial settings.