Widely used as a fluorescent tracer in medical and biological applications, it helps visualize blood flow in the retina during angiography, aiding in the diagnosis of eye diseases like diabetic retinopathy and macular degeneration. It is also employed in flow visualization and leak detection in industrial systems due to its bright fluorescence under UV or blue light. In environmental studies, it tracks water movement and detects groundwater contamination. Additionally, it serves as a pH indicator in analytical chemistry and is used in dye-based assays for cell viability and permeability in research laboratories.