Used in cell culture media as a precursor for purine synthesis, supporting the growth of certain hybridoma and bacterial cells that cannot synthesize purines de novo. Commonly applied in HAT selection medium, where it enables nucleotide salvage pathways in hybrid cells. Also serves as a biomarker in medical research for cellular energy metabolism and ischemia, due to its role as an intermediate in adenine degradation. Found in some skincare formulations for potential cell-repair and anti-aging effects, though evidence remains limited.