N,N’-Desethylene Levofloxacin Hydrochloride is primarily used in pharmaceutical research, especially for studying structural modifications of fluoroquinolone antibiotics to evaluate biological activity, cellular toxicity, and absorption in the body. It also serves as a reference standard in the quality analysis of levofloxacin during drug manufacturing to monitor and control impurities or byproducts formed during synthesis. This compound is essential for quality assurance and ensuring the safety of pharmaceutical products.