This compound is primarily used in peptide synthesis as a protected amino acid derivative. It serves as a building block for constructing complex peptide chains, where the fluorenylmethoxycarbonyl (Fmoc) and tert-butoxycarbonyl (Boc) groups act as orthogonal protective groups for the two nitrogen atoms of the piperazine ring. Its application is crucial in solid-phase peptide synthesis (SPPS), enabling the stepwise assembly of peptides with high precision. The Fmoc group can be selectively removed under mild basic conditions, while the Boc group requires acidic conditions, allowing for controlled deprotection during synthesis. This compound is particularly valuable in producing peptides for pharmaceutical research, drug development, and biochemical studies.