H-Leu-Val-OH, a dipeptide composed of leucine and valine, is primarily used in biochemical research to study peptide interactions and enzyme mechanisms. It serves as a substrate for proteolytic enzymes, aiding in the investigation of enzyme specificity and kinetics. Additionally, it is utilized in the development of peptide-based drugs and biomaterials, where its structural properties are explored for potential therapeutic applications. In the field of nutrition, it is studied for its role in protein synthesis and muscle metabolism, contributing to research on dietary supplements and athletic performance. Its simple structure also makes it a valuable tool in peptide synthesis and sequencing techniques.