This compound is primarily used in peptide synthesis as a protected amino acid derivative. It serves as a building block in solid-phase peptide synthesis (SPPS), where the fluorenylmethoxycarbonyl (Fmoc) group acts as a protecting group for the amino functionality. The m-tolyl group in the structure provides steric and electronic properties that can influence the peptide's conformation and interactions. It is particularly useful in the synthesis of complex peptides and proteins, enabling precise control over the sequence and structure. Additionally, its application extends to the development of peptide-based drugs, where specific modifications are required to enhance stability, bioavailability, or target binding affinity.