Fmoc-3,4-difluoro-L-homophenylalanine is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. Its Fmoc protecting group allows for selective deprotection, enabling the step-by-step construction of peptides with high precision. The incorporation of difluoro groups in the homophenylalanine structure enhances the peptide's stability and bioactivity, making it valuable in the development of pharmaceutical compounds. It is often employed in the synthesis of peptide-based drugs, enzyme inhibitors, and bioactive peptides targeting specific receptors. Additionally, its unique structure can improve the pharmacokinetic properties of peptides, such as metabolic stability and membrane permeability, making it a crucial building block in medicinal chemistry and drug discovery.