Lactate oxidase is widely used in the biomedical field for the detection and measurement of lactate levels in various biological samples, such as blood, serum, and saliva. It plays a critical role in clinical diagnostics, particularly in monitoring conditions like lactic acidosis, sepsis, and hypoxia. Additionally, it is employed in sports medicine to assess athletes' performance and recovery by analyzing lactate accumulation during exercise. In the food industry, lactate oxidase is utilized for quality control, ensuring the freshness and safety of dairy products and fermented foods by measuring lactate content. It also finds application in biosensors and enzyme-based assays for rapid and accurate lactate quantification in research and industrial settings.