Percoll is widely used in biological and medical research for the isolation and purification of cells, organelles, and viruses through density gradient centrifugation. Its low viscosity and biocompatibility make it ideal for separating live cells with minimal damage. It is commonly applied in isolating specific cell types such as hepatocytes, sperm cells, and immune cells like lymphocytes and monocytes. Percoll gradients are also used in microbiology to purify bacteria, mitochondria, and chloroplasts based on their buoyant density. Due to its ability to form stable gradients, it supports high-resolution separation, which is essential in functional studies and downstream applications such as cell culture, flow cytometry, and transplantation experiments.