Fructo-oligosaccharides are widely used as prebiotics in the food and beverage industry to promote the growth of beneficial gut bacteria, improving digestive health. They are commonly added to functional foods, such as yogurts, cereals, and health drinks, to enhance their nutritional value. In the dietary supplement sector, they are included in probiotics to support gut microbiome balance and boost immunity. Additionally, they serve as low-calorie sweeteners in sugar-free and diabetic-friendly products due to their minimal impact on blood glucose levels. Their ability to improve mineral absorption, particularly calcium and magnesium, makes them valuable in products targeting bone health. Fructo-oligosaccharides are also used in animal feed to enhance gut health and nutrient absorption in livestock and pets.