Chitotriose trihydrochloride is primarily used in biomedical and pharmaceutical research due to its bioactive properties. It serves as a key building block in the development of chitosan-based drug delivery systems, enhancing the solubility and stability of therapeutic compounds. Its structure mimics natural oligosaccharides, making it valuable in studying immune modulation and pathogen inhibition. It is also employed in tissue engineering, where it promotes cell adhesion and supports scaffold materials for regenerative medicine. Additionally, chitotriose trihydrochloride is used in cosmetic formulations for its moisturizing and skin-conditioning effects, leveraging its biocompatibility and low toxicity. Research applications include enzyme substrate studies, particularly for chitinase activity assays, aiding in the development of antifungal agents and diagnostic tools.