Corn steep liquor is widely used in the fermentation industry as a nutrient-rich substrate for the growth of microorganisms. It serves as an economical source of nitrogen, vitamins, and minerals in the production of antibiotics, enzymes, and organic acids such as lactic acid and citric acid. In the biotechnology sector, it is utilized to enhance the yield of microbial cultures, particularly in the synthesis of amino acids like lysine and glutamic acid. Additionally, it finds application in the cultivation of yeast for baking and brewing processes, providing essential nutrients for optimal growth. Its use extends to bioethanol production, where it supports the fermentation of sugars by yeast or bacteria. In agriculture, corn steep liquor is employed as a component in animal feed, offering a cost-effective supplement to improve nutritional value. It is also used as a biostimulant to promote plant growth and improve soil quality. Its versatility and affordability make it a valuable resource in various industrial, biotechnological, and agricultural applications.