Cytidine 5'-(tetrahydrogen triphosphate) is widely used in molecular biology and biochemistry for RNA synthesis. It serves as a substrate for RNA polymerases during transcription, enabling the creation of RNA molecules from DNA templates. This compound is also essential in studies of enzymatic mechanisms, particularly those involving RNA synthesis and processing. Additionally, it plays a role in metabolic pathways, contributing to nucleotide biosynthesis and energy transfer processes in cells. Its applications extend to research in virology, where it aids in understanding viral replication and RNA-dependent RNA polymerase activity.