Guanosine 5-triphosphate disodium salt is widely used in biochemical research as a critical energy source and substrate for enzymatic reactions. It plays a vital role in nucleic acid synthesis, serving as a building block for RNA during transcription. Additionally, it is essential in protein synthesis, where it provides energy for the elongation of polypeptide chains. This compound is also utilized in signal transduction pathways, acting as a substrate for G-proteins, which regulate cellular responses to external stimuli. In molecular biology, it is often employed in PCR and other amplification techniques to ensure efficient DNA replication. Furthermore, it is used in studies involving cell metabolism, energy transfer, and enzyme kinetics, providing insights into cellular functions and mechanisms. Its stability and solubility make it a preferred choice for in vitro experiments and diagnostic assays.