Uridine 5-diphosphate disodium salt is widely used in biochemical research as a substrate or precursor in enzymatic reactions, particularly in studies involving glycosyltransferases. It plays a critical role in the biosynthesis of glycoconjugates, such as glycoproteins and glycolipids, by serving as a donor of sugar moieties. This compound is also utilized in the synthesis of nucleotides and nucleic acids, making it valuable in molecular biology and genetic engineering. Additionally, it is employed in metabolic studies to investigate carbohydrate metabolism and nucleotide sugar pathways. Its applications extend to drug development, where it aids in the design and testing of enzyme inhibitors targeting glycosylation processes.