2'-O-Methyl-5-Methyl-U CE phosphoramidite is primarily used in the synthesis of modified oligonucleotides, particularly in the field of nucleic acid research and therapeutic development. It is a key building block for creating RNA molecules with specific modifications, enhancing their stability and resistance to nuclease degradation. This chemical is widely employed in the development of antisense oligonucleotides, siRNA, and other RNA-based therapeutics, where precise control over RNA structure and function is critical. Its application extends to studying gene expression, gene silencing, and the development of targeted treatments for genetic disorders and diseases. Additionally, it is utilized in diagnostic tools and research probes to investigate RNA interactions and mechanisms.