2',3',5'-Tri-O-acetyluridine is primarily used as an intermediate in the synthesis of nucleoside analogs, which are crucial in the development of antiviral and anticancer drugs. Its acetylated groups protect the hydroxyl functions during chemical reactions, allowing for precise modifications to the uridine structure. This compound is also employed in the preparation of modified RNA molecules for research purposes, enabling studies on RNA function and stability. Additionally, it serves as a key building block in the production of labeled nucleosides for use in biochemical assays and diagnostic tools.