This chemical is primarily used in the synthesis of oligonucleotides, particularly in the field of genetic research and therapeutic development. It serves as a key building block in the solid-phase synthesis of modified DNA strands, enabling the incorporation of difluoro-deoxycytidine into oligonucleotide sequences. This modification enhances the stability and binding affinity of the synthesized DNA, making it valuable for applications such as antisense therapy, gene silencing, and the development of diagnostic probes. Its phosphoramidite form allows for efficient and precise coupling during automated DNA synthesis, ensuring high yields and accuracy in the production of custom oligonucleotides.