Widely used in oligonucleotide synthesis, this compound serves as a key building block for the preparation of modified RNA sequences. Its primary application lies in the solid-phase synthesis of RNA for research and therapeutic purposes, particularly in the development of antisense oligonucleotides, siRNA, and mRNA vaccines. The silyl and DMT protecting groups ensure selective reactivity and high coupling efficiency during automated synthesis, while the phosphoramidite group enables rapid and reliable phosphodiester bond formation. The tert-butylphenoxyacetyl group protects the cytosine base, minimizing side reactions and improving overall yield. This reagent is essential for producing high-purity, long-chain RNA with precise modifications, making it critical in pharmaceutical development and molecular biology research.