Used in solid-phase oligonucleotide synthesis to introduce inosine residues with a sulfur-modified backbone linkage. Inosine acts as a universal base in hybridization, allowing for broad pairing flexibility with adenine, cytosine, and thymine, making it valuable in degenerate probe design, SNP detection, and multiplex PCR applications. The 3'-thio modification enhances nuclease resistance in the final oligonucleotide, improving stability for diagnostic and therapeutic uses. The DMT (dimethoxytrityl) group enables real-time monitoring of coupling efficiency during synthesis. The phosphoramidite group ensures high coupling yields under standard DNA synthesizer conditions. Commonly applied in the development of antisense oligonucleotides, molecular beacons, and primers for challenging templates where wobble base pairing is advantageous.