3-Deazaadenosine is primarily utilized in biochemical research as a tool to study cellular processes involving adenosine and its analogs. It is often employed to inhibit enzymes like S-adenosylhomocysteine hydrolase, which plays a role in methylation reactions. By blocking this enzyme, researchers can investigate the impact of disrupted methylation pathways on cellular function and gene expression. Additionally, it has been used in studies related to viral replication, as it can interfere with the metabolism of nucleotides essential for viral growth. In cancer research, 3-Deazaadenosine is explored for its potential to modulate epigenetic mechanisms, offering insights into therapeutic strategies targeting abnormal cell proliferation. Its applications also extend to neurobiology, where it helps in understanding adenosine receptor signaling and its implications in neurological disorders.