N6-Acetyl-2'-deoxyadenosine is primarily used in biochemical research, particularly in studies related to DNA repair mechanisms and nucleoside metabolism. It serves as a modified nucleoside, helping scientists understand how alterations in DNA bases affect cellular processes. This compound is also utilized in the development of nucleotide analogs for potential therapeutic applications, such as antiviral or anticancer drugs. Additionally, it plays a role in investigating enzyme specificity and activity in nucleic acid biochemistry. Its acetyl group makes it a valuable tool for probing the structural and functional dynamics of DNA and its interactions with proteins.