Used extensively in oligonucleotide synthesis, particularly in the production of modified RNA and DNA sequences. It serves as a protected nucleoside, where the trityl group safeguards the 5'-hydroxyl and the benzoyl group protects the 4-amino group of cytidine. This protection is crucial during solid-phase synthesis to prevent unwanted reactions and ensure the correct assembly of nucleotides. It is a key intermediate in the development of therapeutic oligonucleotides, including antisense drugs and siRNA, which are employed in gene silencing and targeted gene therapy. Additionally, it finds applications in research for studying nucleic acid interactions and designing novel biomolecules.