Used primarily in oligonucleotide synthesis, this compound serves as a protected form of 5-methyl-2'-deoxycytidine. The 4,4'-dimethoxytrityl (DMT) group protects the 5'-hydroxyl, while the isobutyryl group safeguards the exocyclic amine, ensuring selective reactions during DNA strand assembly. It is particularly valuable in automated solid-phase synthesis, enabling the production of custom DNA sequences for research, diagnostics, and therapeutic applications. Its stability and ease of deprotection make it a key reagent in molecular biology and genetic engineering workflows.