Used extensively in cell biology research to track DNA synthesis and cell proliferation. It incorporates into newly synthesized DNA during the S-phase of the cell cycle, allowing precise labeling and detection of dividing cells. Often used as an alternative to bromodeoxyuridine (BrdU), it offers higher sensitivity and does not require DNA denaturation for detection when used with click chemistry. Commonly applied in studies involving stem cells, cancer research, and developmental biology to monitor cell division and lineage tracing.