Ribonuclease H from Escherichia coli is widely used in molecular biology research for its ability to specifically degrade the RNA strand in RNA-DNA hybrids. This enzyme is essential in studying reverse transcription processes, as it helps in removing RNA templates during cDNA synthesis. It is also utilized in various techniques like RT-PCR and DNA sequencing to improve accuracy and efficiency. Additionally, Ribonuclease H plays a critical role in the development of antisense oligonucleotide therapies, where it aids in the degradation of target RNA molecules, enabling gene silencing. Its application extends to the study of DNA replication and repair mechanisms, providing insights into cellular processes and potential therapeutic targets.