n-Hexadecyl β-D-maltoside is widely used in biochemical research as a non-ionic detergent for solubilizing membrane proteins. Its mild nature makes it suitable for maintaining protein stability and function during extraction and purification processes. It is particularly valuable in structural biology, where it aids in the crystallization of membrane proteins for X-ray diffraction studies. Additionally, it is employed in the reconstitution of membrane proteins into lipid bilayers for functional studies. In cell biology, it is used to permeabilize cell membranes without disrupting cellular structures, facilitating the study of intracellular components. Its application extends to the pharmaceutical industry, where it is used in drug delivery systems to enhance the solubility and bioavailability of hydrophobic drugs.