n-Octyl-β-D-thioglucopyranoside is widely used in biochemical research as a detergent for solubilizing membrane proteins. It effectively disrupts lipid bilayers, making it valuable for extracting integral membrane proteins while maintaining their native structure and function. This property is crucial for studies involving protein purification, crystallization, and functional analysis. Additionally, it is employed in the reconstitution of membrane proteins into artificial lipid systems, aiding in the study of their interactions and mechanisms. Its mild nature and ability to preserve protein activity make it a preferred choice in various experimental setups, including electrophoresis and immunoassays.