Aflatoxin B1 is primarily used in research settings to study its effects on biological systems, particularly its role as a potent carcinogen. It is extensively employed in toxicology studies to understand the mechanisms of liver damage and cancer development. Researchers use it to investigate the impact on DNA, RNA, and protein synthesis, as well as to explore potential detoxification pathways. Additionally, it serves as a critical tool in developing and testing methods for detecting and mitigating aflatoxin contamination in food and agricultural products. Its application is also significant in evaluating the efficacy of treatments or preventive measures against aflatoxin-induced health risks.