Endonuclease AscI is widely used in molecular biology for DNA manipulation. It is particularly valuable in genetic engineering for creating precise cuts in DNA sequences, enabling the insertion, deletion, or modification of specific genes. This enzyme is essential in cloning experiments, where it helps in the preparation of DNA fragments for ligation into vectors. Additionally, AscI is utilized in genome editing and the construction of recombinant DNA molecules, facilitating advanced research in biotechnology and genetic studies. Its specificity and efficiency make it a key tool in the development of genetically modified organisms and the study of gene function.