Used in fluorescence microscopy to label mitochondria in live cells. It accumulates in active mitochondria due to membrane potential, allowing visualization of mitochondrial structure and dynamics. Commonly applied in studies of mitochondrial function, cell health, apoptosis, and metabolic activity. Compatible with live-cell imaging, it can be used alongside other fluorescent markers for co-localization studies. Also utilized in flow cytometry to assess mitochondrial mass and membrane potential in cell populations.