DL-3,4-Dihydroxymandelic Acid is primarily used in biochemical research as a metabolite in the study of catecholamine metabolism. It plays a significant role in understanding the pathways and mechanisms of neurotransmitters like dopamine, norepinephrine, and epinephrine. Researchers utilize it to investigate oxidative stress and its impact on neurological disorders. Additionally, it serves as a reference standard in analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry to quantify and identify related compounds in biological samples. Its applications extend to studies on antioxidant activity and its potential role in mitigating cellular damage caused by free radicals.