Alpha-cyanocinnamic acid is widely used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry as a matrix compound. It helps in the ionization of analytes, particularly peptides, proteins, and other biomolecules, enabling their detection and analysis. This chemical is favored for its ability to absorb UV light efficiently, which facilitates the desorption and ionization process. Additionally, it is employed in the study of small molecules and polymers due to its compatibility with a wide range of analytes. Its application extends to research in proteomics, metabolomics, and material science, where precise molecular analysis is crucial.