2,4-Dihydroxycinnamic acid is widely used in the field of analytical chemistry, particularly in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It serves as an effective matrix for the analysis of various biomolecules, including peptides, proteins, and polymers, due to its ability to absorb UV light and facilitate the ionization process.

In the pharmaceutical industry, it is studied for its potential antioxidant and anti-inflammatory properties, making it a candidate for the development of therapeutic agents. Additionally, it is explored in cosmetic formulations for its ability to protect skin from oxidative stress and UV-induced damage.

In agriculture, 2,4-Dihydroxycinnamic acid is investigated for its role in plant growth regulation and its potential to enhance stress resistance in crops. Its derivatives are also used in the synthesis of organic compounds and as intermediates in chemical research.