Phospho-Protein Extraction Buffer II is widely used in molecular biology and biochemistry research for the efficient extraction of phosphorylated proteins from cells and tissues. Its primary application is in the study of cell signaling pathways, where it helps isolate phospho-proteins while maintaining their phosphorylation state. This buffer is particularly useful for downstream applications such as Western blotting, immunoprecipitation, and mass spectrometry, enabling researchers to analyze protein phosphorylation and its role in cellular processes. Its optimized formulation ensures minimal protein degradation, making it a reliable tool for investigating post-translational modifications and signaling mechanisms.