Used primarily in organic synthesis, 2,4-Hexadiyne-1,6-diol serves as a building block for creating complex molecules. Its diacetylene structure makes it valuable in the development of polymers with unique electronic and optical properties. It is also employed in the preparation of specialty chemicals, including liquid crystals and advanced materials for photonics. Additionally, it finds application in research for studying reaction mechanisms involving diynes, contributing to advancements in synthetic chemistry.