Used primarily in research settings as a selective and irreversible inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase. It is widely applied in studies involving signal transduction pathways to block EGFR-mediated cellular responses, such as proliferation, differentiation, and survival. Due to its irreversible binding mechanism, it allows for prolonged suppression of EGFR activity, making it valuable in experiments requiring sustained inhibition. Commonly used in cancer research to investigate the role of EGFR in tumor growth and to evaluate potential therapeutic strategies, especially in models of epithelial cancers. Also employed to distinguish EGFR-dependent processes from those mediated by other kinases, supporting mechanistic studies in cell biology and pharmacology.