N-(2-Aminoethyl)isoquinoline-5-sulfonamide hydrochloride is primarily utilized in biochemical research as a potent inhibitor of protein kinases. It plays a significant role in studying cellular signaling pathways, particularly those involving calcium/calmodulin-dependent protein kinase II (CaMKII). This compound is employed to investigate the mechanisms of kinase activity and its implications in various physiological and pathological processes, such as neuronal function, cardiac regulation, and memory formation. Additionally, it serves as a valuable tool in drug discovery and development, aiding in the identification of potential therapeutic targets for diseases linked to kinase dysregulation, including cancer, neurodegenerative disorders, and cardiovascular conditions. Its application extends to in vitro and in vivo studies, providing insights into the modulation of kinase-related pathways and their impact on cellular behavior.