MK-4101 is primarily utilized in research settings as a potent and selective inhibitor of the enzyme Sphingosine Kinase 1 (SphK1). This enzyme plays a crucial role in the sphingolipid metabolism pathway, which is involved in various cellular processes, including cell growth, survival, and inflammation. By inhibiting SphK1, MK-4101 helps researchers study the effects of disrupted sphingolipid signaling in diseases such as cancer, inflammatory disorders, and autoimmune conditions. Its application in preclinical studies aids in understanding the potential therapeutic benefits of targeting SphK1 in these diseases. Additionally, MK-4101 is used to explore the role of sphingolipids in cellular signaling pathways, contributing to the development of novel treatment strategies.