DEAE-Sephadex is widely used in biochemistry and molecular biology for the separation and purification of biomolecules, particularly proteins and nucleic acids. Its anion-exchange properties make it effective for binding negatively charged molecules, allowing for their isolation from complex mixtures. It is commonly employed in column chromatography, where it facilitates the separation of proteins based on their charge differences. DEAE-Sephadex is also utilized in the purification of enzymes, antibodies, and other biologically active compounds. Additionally, it finds application in desalting processes, where it helps remove salts and other small molecules from protein solutions. Its versatility and ease of use make it a valuable tool in research laboratories and industrial settings for downstream processing and analytical applications.