DEAE–Sepharose is widely used in chromatography for the purification of proteins, nucleic acids, and other biomolecules. Its application is particularly prominent in anion exchange chromatography, where it separates molecules based on their charge differences. It is commonly employed in the purification of enzymes, antibodies, and plasma proteins, making it a valuable tool in biochemical and biopharmaceutical research. Additionally, DEAE–Sepharose is utilized in the isolation of specific DNA or RNA fragments, aiding in molecular biology studies. Its high capacity and stability make it suitable for both laboratory-scale and industrial-scale purification processes.