DEAE–Sepharose is widely used in chromatography for the purification of proteins, nucleic acids, and other biomolecules. Its anion-exchange properties make it effective for separating molecules based on their charge. It is commonly employed in the isolation of enzymes, antibodies, and plasma proteins in biochemical and biopharmaceutical research. The matrix is also utilized in the removal of contaminants during downstream processing. Its high capacity and stability make it suitable for both laboratory-scale and industrial applications. Additionally, DEAE–Sepharose is often used in the fractionation of complex biological samples, aiding in the study of molecular interactions and functions.