Methyl 5-iodo-1H-indole-3-carboxylate is primarily used in organic synthesis as a key intermediate for the preparation of various indole derivatives. These derivatives are valuable in pharmaceutical research, particularly in the development of compounds with potential biological activities, such as anticancer, antiviral, and anti-inflammatory agents. The iodine substituent at the 5-position makes it a versatile building block for further functionalization through cross-coupling reactions, such as Suzuki or Sonogashira couplings, enabling the creation of complex molecules. Additionally, it is employed in the synthesis of natural product analogs and bioactive compounds, contributing to drug discovery and medicinal chemistry efforts. Its role in constructing indole-based frameworks is crucial for exploring new therapeutic agents.